We prepared Ni nanoparticle-deposited silica (SiO2) microspheres using a method based on the collision of Ni nanoparticles, which were generated by hydrogen reduction of NiCl2, with SiO2 microspheres. SiO2 microspheres were heated in a gas-phase reactor to a temperature sufficiently high to soften their surfaces. Notably, when SiO2 microspheres were heated to temperatures higher than 973 K before collision with Ni nanoparticles, the nanoparticles showed no signs of sintering on the SiO2 microsphere surfaces during heat treatment at a high temperature of 1,073 K. In addition, we independently controlled the diameter and content of Ni nanoparticles on the SiO2 microspheres by changing NiCl2 evaporation temperature and NiCl2-carrier gas flow rate.