Aim: Effects of two cosurfactants, n-alkylglycosides with mono- or disaccharide groups ? N-nonyl ?-D-glucopyranoside (N-Glu) and N-decyl ?-D-maltoside (D-Mal) ? were studied to the stability in saline solution, interaction with serum albumin, and blood circulation of the lipid nanoemulsion (LNE). Methods: The LNEs composed of soybean oil, phosphatidylcholine, and sodium palmitate were prepared without (Control-LNE) and with N-Glu or D-Mal (NG-LNE and DM-LNE, respectively). Results: In saline solution, NG-LNE exhibited a smaller droplet size than Control-LNE, while the size of DM-LNE was significantly increased compared with the other LNEs. The fluorescence resonance energy transfer method showed that the order of albumin interaction was DM-LNE?>?NG-LNE?>?Control-LNE. In vivo blood circulation in mice, showed greater fractions of both NG-LNE and DM-LNE remaining in blood over time compared with Control-LNE. Conclusions: The nature of high stability in saline solution and high affinity for serum albumin led to the prolonged circulation of LNE.