The self-assembly of the two anomeric forms of n-hexadecyl-D-maltopyranoside (denoted alpha-C(16)G(2) and beta-C(16)G(2)) has been studied in dilute aqueous solution by means of surface tension measurements, scattering methods (dynamic light scattering, static light scattering, and small-angle X-ray and neutron scattering), and cryo-transmission electron microscopy at different surfactant concentrations and temperatures. Surface tension measurements demonstrate differences in the surfactant adsorption at the air-water interface, where alpha-C(16)G(2) shows a lower CMC than beta-C(16)G(2). Similarly, micelle morphology was found to profoundly depend on anomerism. beta-C(16)G(2) preferentially forms very elongated micelles with large persistence lengths, whereas alpha-C(16)G(2) assembles into smaller micelles for which the structure varies with concentration and temperature. The differences between the two surfactant anomers in terms of self-assembly can be attributed to the interaction between neighboring headgroups. Specifically, beta-C(16)G(2)allows for a closer packing in the palisade layer, hence reducing the micelle curvature and promoting the formation of more elongated micelles. Strong intermolecular headgroup interactions may also account for the observed rigidity of the micelles.