This paper proposes a new method to improve the reverse flotation performance of magnetite ore using a pulse magnetic field. Flotation tests were performed in a flotation column with six external coils, which created the pulse magnetic field. The preferred parameters of the pulse magnetic field were identified, and the results were compared with those obtained using a steady or no magnetic field. The results showed that the pulse magnetic field increased the recovery and grade of concentrate Fe by 1.12% and 4.22%, respectively, compared to the corresponding values obtained using no magnetic field. The concentrate Fe grade obtained using the pulse magnetic field was better than that obtained using a steady magnetic field, indicating that the presence of the pulse magnetic field caused the selectivity to depress the magnetite particles to be high. The magnetic field intensity, switching frequency, and on/off time ratio were critical parameters affecting the magnetic flotation efficiency, and the settings for these three parameters should be properly selected for improving the magnetic flotation performance.