The study of phosphatases continues to flourish given their prominence in signal transduction pathways and the regulation of cell function. Our research efforts in this area focus on the potent inhibition of serine/threonine phosphatases, PP1 and PP2A, by a structurally diverse class of natural products. We herein report the completed total synthesis of the serine/threonine phosphatase inhibitor calyculin C (1) as part of an ongoing effort to elucidate key structural requirements for phosphatase inhibition by the aforementioned diverse class of natural products. Synthetic issues addressed include () the remote protecting group effect on Brown crotylboration diastereoselectivity during the introduction of the C-10-C-11 stereocenters and (2) the formation of the C-25-C-26 double bond using a fully deprotected C-26-C-37 phosphonium salt (3). In addition,the concurrent synthesis of R-34-calyculin C (29) clarified our previous C-34-stereochemical assignment of C26-C37 phosphonium salts (3 and 27) (Ogawa, A. K.; DeMattei, J. A.; Scarlato, G. R.; Tellew, J. E.; Chong, L. S.; Armstrong, R. W. J. Org. Chem. 1996, 61, 6153).