The conventional electrodes of direct methanol fuel cells (DMFCs) usually encounter a problem that the catalysts sink into the diffusion layer after a period of operation, causing a lowered catalyst utilization and degraded cell performance. Aiming to alleviate this problem, in this work a novel anode electrode structure is proposed, in which a micro porous layer containing Nafion polymer is added between the catalyst layer and the microporous layer with PTFE. The presence of the Nafion-contained layer can expand the three-phase interface region of the electrochemical reactions and improve the utilization of the catalyst. The single cell test showed that the peak power densities of the novel membrane electrode assembly (MEA) fed with 0.5 M and 2 M methanol solutions reached 38.35 mW cm(-2) and 101.82 mW cm(-2), which increased by 100.42% and 15.27% compared with those of conventional single microporous layer. Electrochemical impedance spectroscopy (EIS) measurements indicated the charge transfer resistance of the conventional MEA structure was increased by 303.78%, while the new one was decreased by 47.91% after continuously operating for 48 h. The anode electrochemical active surface area (ECSA) values of the novel MEA and the conventional MEA were 52.6 m(2) g(-1) and 44.3 m(2) g(-1). These experimental results showed that the performance of the double microporous layer MEA was higher than that of the conventional MEA. This new microporous layer structure is promising to be used in fuel cells to improve cell performance and alleviate performance degradation after long-term operating. (C) 2019 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.