This study aimed to compare the microshear bond strength (MSBS) of three universal adhesives and a three-step conventional adhesive to dentin after 24-hour and one-year storage in water. A new fluoride-releasing universal adhesive (Clearfil Universal Bond Quick: CUQ) and two commercially available adhesives (ScotchBond Universal: SBU and All-Bond Universal: ABU) were evaluated with phosphoric acid etching (PA-etch mode) or without it (self-etch mode). All-Bond 3 (AB3) served as control group. After bonding composite cylinders to dentin discs obtained from caries-free human teeth, the specimens were stored in deionized water at 37 degrees C for either 24 hours or one year (n = 14) before MSBS measurement. Two-way ANOVA analysis of the results showed that the adhesives, storage time and their interactions had a significant effect on MSBS (p < 0.01). In self-etch mode, there was no significant difference among universal adhesives at the baseline. In PA-etch, the CUQ and SBU showed significantly higher MSBS compared with AB3 (p < 0.05). At baseline, no difference was found between the two modes for each universal adhesive (p > 0.05). After one year, CUQ in self-etch mode showed a slight increase in nominal MSBS (p > 0.05) and Weibull characteristic strength, which was significantly higher than SBU and ABU in the corresponding mode. There was no difference among the three universal adhesives in PA-etch mode after one year (p > 0.05). In conclusion, the durability and reliability of dentin bonding with universal adhesives in different application modes depended on the material; and the self-etch approach showed promising results for the tested fluoride-releasing universal adhesive.