The solubility of chloramphenicol in pure supercritical fluid carbon dioxide (SF-CO2) has been determined using a continuously stirred supercritical solubility vessel (SSV) directly interfaced with online supercritical fluid chromatography (SFC) using a single valve interface. Solubility measurements were performed at two temperatures of 313 and 333 K with pressures ranging from 10 to 26 MPa. For each individually loaded sample, a series of increasingly more saturated chloramphenicol solutions were prepared in situ by ramping the SF-CO2 pressure within the SSV in 2 or 3 MPa steps. Once equilibrium dissolution was established at each pressure step value, online SFC solubility measurements were performed. The combined SSV-SFC instrumentation and methodology enabled the rapid acquisition of chloramphenicol SF-CO2 solubility data. Chloramphenicol exhibited limited solubility in SF-CO2, providing mole fraction solubility values of 4.9 x 10(-6) to 9.9 x 10(-6) within the experimental range studied. All sets of SSV-SFC experimental solubility values obtained for chloramphenicol were successfully correlated in accordance with the semiempirical SF-CO2 density-based models developed by (i) Mendez-Santiago and Teja (MST) and (ii) Bartle.