The process of L-proline permeate desalting by electrodialysis with subsequent separation of L-proline from accompanying amino acids was developed. The overall yield of L-proline was 87.3%. The changes of dry substances concentration, pH, solutions electroconductivity in the course of electrodialysis were presented. It has been established that the method used minimizes the losses of L-proline (<4%), purified the permeate from mineral ions (>98.6%), and from basic and acidic amino acids. The process of L-proline separation from accompanying amino acids is based on the ability of primary amino acids to form Schiff bases with 5-sulfosalicylic aldehyde on the porous anion exchanger.