Enzymatically Crosslinkable Hyaluronic Acid-Gelatin Hybrid Hydrogels as Potential Bioinks for Tissue Regeneration

Phuong Le Thi; Joo Young Son; Yunki Lee; Seung Bae Ryu; Kyung Min Park; Ki Dong Park

Macromolecular Research, Vol.28, No.4, 400-406, April, 2020

DOI10.1007/s13233-020-8052-x Export Citation

Enzymatically Crosslinkable Hyaluronic Acid-Gelatin Hybrid Hydrogels as Potential Bioinks for Tissue Regeneration

Phuong Le Thi, Joo Young Son, Yunki Lee, Seung Bae Ryu, Kyung Min Park¹, and Ki Dong Park^†

Department of Molecular Science and Technology, Ajou University, Suwon 16499, Korea
¹Department of Bioengineering and Nano-Bioengineering, Incheon National University, 119 Academy-ro, Yeonsu-gu, Incheon 22012, Korea

E-mail:

Hydrogels that mimic the composition and properties of the extracellular matrix have attracted great attention as potential polymeric scaffolds for tissue engineering applications. In this study, an injectable hydrogel composed of hyaluronic acid and gelatin was developed via the oxidative coupling reaction using horseradish peroxidase (HRP). The hydrogel was prepared by mixing two phenol-conjugated polymer solutions, hyaluronic acid-tyramine (HA-TA) and gelatin-hydroxyphenyl propionic acid (GH), in the presence of HRP and hydrogen peroxide (H202). The gelation rate and mechanical properties of composite hydrogel were controlled by adjusting the HRP and H202 concentrations, respectively Compared to the pure HA-TA hydrogels, the stability and cellular behaviors of composite hydrogel improved significantly In addition, the injectable hydrogel system showed good performance in 3D printing with high cell viability after one day of printing. The results suggest that enzymatically crosslinked HA-TA and GH hybrid (HA-TA/GH) composite of HA-TA and GH hydrogel has the potential as a material for tissue engineering and 3D printing biofabrication.

Keywords:injectable hydrogels;hyaluronic acid;gelatin;tissue regeneration

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[Referenced By]

[1] Ahadian S, Sadeghian RB, Salehi S, Ostrovidov S, Bae H, Ramalingam M, Khademhosseini A, Bioconjugate Chem., 26, 1984 (2015)

[2] Calo E, Khutoryanskiy VV, Eur. Polym. J., 65, 252 (2015)

[3] Ko DY, Shinde UP, Yeon B, Jeong B, Prog. Polym. Sci, 38, 672 (2013)

[4] Liu Y, He W, Zhang Z, Lee B, Gels, 4, 46 (2018)

[5] Kharkar PM, Kiick KL, Kloxin AM, Chem. Soc. Rev., 42, 7335 (2013)

[6] Fiorica C, Palumbo FS, Pitarresi G, Gulino A, Agnello s, Giammona G, RSC Adv., 5, 19715 (2015)

[7] Park KM, Ko KS, Joung YK, Shin H, Park KD, J. Mater. Chem., 21, 13180 (2011)

[8] Carthew J, Frith JE, Forsythe JS, Truong VX, J. Mater. Chem. B, 6, 1394 (2018)

[9] Wang Z, Tian Z, Menard F, Kim K, Biofabrication, 9, 044101 (2017)

[10] Lee F, Chung JE, Kurisawa M, Soft Matter, 4, 880 (2008)

[11] Eke G, Mangir N, Hasirci N, MacNeil S, Hasirci V, Biomaterials, 129, 188 (2017)

[12] Liu CX, Bae KH, Yamashita A, Chung JE, Kurisawa M, Biomacromolecules, 18(10), 3143 (2017)

[13] Park S, Park KM, Biomaterials, 182, 234 (2018)

[14] Wu S, Deng L, Hsia H, Xu K, He Y, Huang Q, Peng Y, Zhou Z, Peng C, J. Biomater. Appl., 10, 1380 (2017)

[15] Teixeira LS, Feijen J, van Blitterswijk CV, Dijkstra PJ, Karperien M, Biomaterials, 33, 1281 (2012)

[16] Fan Z, Zhang Y, Fang S, Xu C, Li X, RSC Adv., 5, 1929 (2015)

[17] Bae JW, Choi JH, Lee Y, Park KD, J. Tissue Eng. Regen. M., 9, 1225 (2015)

[18] Khanmohammadi M, Dastjerdi MB, Ai A, Ahmadi A, Godarzi A, Rahimi A, Ai j, Biomater. Sci., 6, 1286 (2018)

[19] Sakai S, Nakahata M, Chem. Asian J., 12, 3098 (2017)

[20] Lee F, Bae KH, Kurisawa M, Biomed. Mater., 11, 014101 (2015)

[21] Ganesh N, Hanna C, Nair SV, Nair LS, Int. J. Biol. Macromol., 55, 289 (2013)

[22] Ying H, Zhou J, Wang M, Su D, Ma Q, Lv G, Chen J, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 101, 487 (2019)

[23] da Silva MA, Bode F, Drake AF, Goldoni S, Stevens MM, Dreiss CA, Macromol. Biosci., 14, 817 (2014)

[24] Thi TTH, Lee Y, Thi PL, Park KD, Macromol. Res., DOI 10.1007/s13233-019-7115-3 (2019).

[25] Jin R, Teixeira LSM, Dijkstra PJ, van Blitterswijk CA, Karperien M, Feijen J, J. Control. Release, 152, 186 (2011)

[26] Chen F, Yu S, Liu B, Ni Y, Yu C, Su Y, Zhu X, Yu X, Zhou Y, Yan D, Sci. Rep., 6, 20014 (2016)

[27] Tran NQ, Joung YK, Lih E, Park KM, Park KD, Macromol. Res., 19(3), 300 (2011)

[28] Choi SM, Lee Y, Son JY, Bae JW, Park KM, Park KD, Macromol. Res., 25(1), 85 (2017)

[29] Lee Y, Bae JW, Oh DH, Park KM, Chun YW, Sung HJ, Park KD, J. Mater. Chem. B, 1, 2407 (2013)

[30] Kurisawa M, Chung JE, Yang YY, Gao SJ, Uyama H, Chem. Commun., 4312 (2005).

[31] Wang LS, Du C, Chung JE, Kurisawa M, Acta Biomater., 8, 1826 (2012)

[32] Jin R, Teixeira LSM, Dijkstra PJ, Karperien M, van Blitterswijk CA, Zhong ZY, Feijen J, Biomaterials, 30, 2544 (2009)

[33] Jin R, Lou B, Lin C, Polym. Int., 62, 353 (2013)

[34] Ghobril C, Grinstaff MW, Chem. Soc. Rev., 44, 1820 (2015)

[35] Ramadhan W, Kagawa G, Hamada Y, Moriyama K, Wakabayashi R, Minamihata K, Goto M, Kamiya N, ACS Appl. Bio Mater., 2, 2600 (2019)

[36] Silva CR, Babo PS, Gulino M, Costa L, Oliveira JM, Silva-Correia JJ, Domingues RMA, Reis RL, Gomes ME, Acta Biomater., 77, 155 (2018)

[37] Oliveira JM, Carvalho L, Silva-Correia J, Vieira S, Majchrzak M, et al., NPJ Regen. Med., 3, 8 (2018)

[38] Lin Z, Wu M, He H, Liang Q, Hu C, Zeng Z, Cheng D, Wang G, Chen D, Pan H, Ruan C, Adv. Funct. Mater., 29, 180843 (2019)

[39] Xu C, Lee W, Dai G, Hong Y, ACS Appl. Mater. Interfaces, 10, 9969 (2018)