In this present work, amorphous silica is synthesized by simple solution method using sodium silicate (Na2SiO3) as raw material. The synthesized silica is dispersed in various dispersing agent obtained from local paint industry and used for painting application. XRD analysis revealed the existence of amorphous silica with a peak at 2 theta value of 23 degrees and the SEM analysis exemplified silica nanoparticles demonstrating spherical morphology with agglomeration. Different dispersing agents (as indicated by the codes given by paint industry) were used for the dispersion of SiO2 by beads milling process and its effects were studied. Among the several dispersing agents used amorphous silica dispersed in SND 504 (Sodium salt of polymeric carboxylic acid with water) dispersing agent exhibit better dispersion compared to the other dispersing agents. Further, 10 wt% of SND 504 dispersing agent was optimized with the particle size to 384 nm and zeta potential value of -24.69 mV. The contact angle measurement of the dispersed silica reveals the superior hydrophobic behaviour of SiO2, especially with 10 wt% SND 504 dispersing agent. The critical surface tension of SiO2 with 10 wt% SND 504 dispersing agent reveal low value compared to other concentration of dispersant. Thus, the dispersed silica nanoparticles with enhanced hydrophobicity can be effectively used for painting applications as fillers. Silica dispersed in 10 wt% SND 504 dispersing agent show superior anti-bacterial activity compared to the bare silica which is also reported. (C) 2019 The Society of Powder Technology Japan. Published by Elsevier B.V. and The Society of Powder Technology Japan. All rights reserved.