The anionic detergent sodium dodecyl benzene sulfonate (SDBS) inhibits anaerobic solid waste fermentation process in mesophilic anaerobic digesters. In this study, the effect SDBS on the performance of anaerobic digestion (AD) of mixture of sewage sludge, food waste, and green waste serving as substrate was investigated. The batch experiments were conducted with five SDBS concentrations namely 0, 0.01, 0.02, 0.05, 0.1, and 0.2 g/g (SDBS/dry sludge) under mesophilic condition (37 +/- 1 degrees C) and lasted for 63 days. The results showed that the presence of SDBS remarkably increased the release of protein and carbohydrate, and resulting in the serious accumulation of volatile fatty acids (VFAs), especially for propionate accumulation. Likewise, the observed variations in enzyme activities associated with different stage of AD revealed that methanogenesis was quite sensitive to SDBS and inhibited by the increase of SDBS addition. Meanwhile, the presence of SDBS decreased the pH value and the concentration of free ammonia, but increased the concentration of NH4+-N. Furthermore, the production of biogas was reduced by SDBS. In conclusion, SDBS addition has a negative impact on anaerobic co-digestion. On the one hand, methanogens were severely inhibited and biogas yield decreased remarkably, on the other hand, the accumulation of VFAs was excessive. Thus, the presence of surfactant (SDBS) in the municipal organic waste should be concerned during the waste disposal via anaerobic digestion process.