As a novel alternative fuel and surrogate component, 2,6,10-trimethyl dodecane has received extensive attention. For designing and optimizing internal combustion engines and propulsion systems by computational fluid dynamics, and providing more choices for a branched surrogate component to develop a more accurate surrogate model, a comprehensive detailed chemical kinetic model for 2,6,10-trimethyl dodecane has been developed based on 35 reaction classes to numerically describe its experimental observations. The proposed detailed kinetic model for 2,6,10-trimethyl dodecane has been validated against a wide range of experimental data, including ignition delay time, flow reactor, and laminar flame speeds. A good agreement between the numerical and the experimental data is observed. Using the kinetic model reduction scheme, high-temperature and low-temperature chemical kinetic models were eventually obtained and validated against the detailed kinetic model. The successful implementation of a kinetic model construction based on 35 reaction classes has indicated the possibility of its application for the development of mechanisms for the larger hydrocarbon fuels with asymmetric and iso-paraffinic molecular structures.