The stator winding acts as a carrier for the electrical load, and its loss accounts for about 50% of the total loss in AC machines. So how to design the stator winding for reducing winding loss is extremely crucial. Some traditional transposition methods are adopted in the winding design for large AC machines. Although they have a certain effect on reducing winding loss, there is still room for the improvement in the winding design and manufacturing technology. In this paper, a new global transposition method of stator winding is proposed. Then a megawatt driving motor is taken as the research object, and the eddy current losses, circulating current losses and total losses of winding strands are calculated by the three dimensional (3-D) finite element method (FEM) which has been validated by the experiment. In addition, by comparison with the two original transposition methods, it is proved that the proposed global transposition method is more effective in further reducing the winding loss, and the winding loss distribution is more uniform. This global transposition method proposed in this paper provides a reference for the optimal deign of the stator winding in AC machines.