Metal-organic frameworks (MOFs), with enormous internal surface areas, ultrahigh porosity, and designable structure consisting of organic and inorganic components, have the potential application value that has not been fully explored in biological sterilization. In this work, the sustained-release sterilization abilities of three different MOFs, that is, [AgL](n)center dot nH(2)O (p-MOF), MOF-5, and ZIF-8, against three typical oral bacteria were studied using sustained-release curve measurement, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide reduction assay, and growth curve determination. Compared with traditional antibacterial materials, such as silver nitrate (AgNO3), the p-MOF material exhibits better slow-release bactericidal properties. The sterilization property of MOFs containing Ag+ ions is better than that of MOFs containing Zn2+ ions. In addition, the sterilization time of MOFs in the solid phase is longer than that of MOFs in the liquid phase, which is ascribed to the slow release rate of metal ions in the frameworks of solid-phase MOFs. Moreover, the sustained-release property of MOFs is strongly associated with their structural characteristics and the corresponding chemical stability. Hopefully, this work can bring new insights into a new competitive antibacterial material against oral bacteria and the rational design of antibacterial MOFs.