In this work, a new bio-electrochemical fuel cell was constructed and the effect of boron was investigated to obtain improved electrogenic activity of a green algae (Choricystis sp.). In a specially designed bio-electrochemical fuel cell, electrode combinations of indium tin oxide (ITO) and a carbon layer are used. Open circuit potential (OCP) measurements of algal cells placed bio-electrochemical fuel cells were performed with a cyclic on-off illumination of a LED light source. The results revealed that 60 mu M is the efficient boron concentration for the algal growth and pigmentation. Higher doses of boron limited the algal growth. However, the algal growth reduced slightly at higher doses, pointing out a possible boron export mechanism in green algae. OCP analysis showed that hydrogen was electrocatalytically reduced at the cathode site and an 18 mV voltage was obtained from borondeficient (0 mu M) Choricystis sp. samples. A significant enhancement in voltage output up to 33 mV was achieved from 60 mu M of boron-treated algal samples. The maximum power density was calculated from the boron-treated Choricystis sp. at pseudo-steady state as 42.2 mW m(-2) at a current density of 154 mA m(-2). (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.