Magnetic imprinted polymer having selectivity for cephalexin has been synthesized and evaluated for extraction of the antibiotic from selected food matrix. Fe3O4 magnetite has been prepared by co-precipitation of FeCl(2 center dot)4H(2)O and FeCl(3 center dot)6H(2)O in 1:2 molar ratios. Imprinted polymer has been synthesized by polymerization of methacrylic acid and ethylene glycol dimethacrylate in the presence of cephalexin over the surface of Fe3O4 magnetite. Selectivity of imprinted polymer over non-imprinted polymer is determined under different solvent conditions. Selectivity in both methanol and acetonitrile is found to be > 35. Selectivity is pH dependent in aqueous buffer solution and is in range of 2.33 to 21.3, maximum being at pH 5.0. Electrostatic interaction between amino group of cephalexin and carboxylic group in polymer appears to be main force contributing to selectivity of imprinted polymer. Cephalexin imprinted magnetic polymer extracted 80, 88, 63 and 78% cephalexin from water, milk, honey and egg white, respectively.