This paper investigates experimentally and numerically the dynamics of solid particles during the layer inversion of binary solid-liquid fluidized beds in narrow tubes. Layer inversion can happen in solid classifiers and biological reactors, where different solid particles coexist and segregation by diameter and density occurs. The fluidized beds were formed in a 25.4 mm-ID pipe and consisted of alumina and aluminum beads with diameters of 6 and 4.8 mm, respectively. We placed initially the lighter particles on the bottom in order to force an inversion of layers, mimicking the layer inversion mechanism. In the experiments, we filmed the bed with a high-speed camera and tracked individual beads along images, while in numerical simulations we computed the bed evolution with a CFD-DEM (computational fluid dynamics - discrete element method) code. We found the distances traveled by individual particles during the inversion and the characteristic time for layer inversion. (C) 2019 Elsevier B.V. All rights reserved.