RAFT/PISA based Ni-NTA polymeric particles for virus-mimetic influenza vaccines

Chaeyeon Lee; Aran Hwang; Leeja Jose; Ji Hyun Park; Sunah Jang; Jae Kwang Song; Yeonji Kim; Yungyeong Cho; Heung Bae Jeon; Jun-O Jin; Hyun-jong Paik

Journal of Industrial and Engineering Chemistry, Vol.86, 35-38, June, 2020

DOI10.1016/j.jiec.2020.03.004 Export Citation

RAFT/PISA based Ni-NTA polymeric particles for virus-mimetic influenza vaccines

Chaeyeon Lee¹, Aran Hwang¹, Leeja Jose¹, Ji Hyun Park², Sunah Jang², Jae Kwang Song², Yeonji Kim³, Yungyeong Cho³, Heung Bae Jeon³, Jun-O Jin^{4, 5, †}, and Hyun-jong Paik^{1, †}

¹Department of Polymer Science & Engineering, Pusan National University, Busan 46241, Republic of Korea
²Research Center for Bio-Based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Republic of Korea
³Department of Chemistry, Kwangwoon University, Seoul 01897, Republic of Korea
⁴Shanghai Public Health Clinical Center, Shanghai Medical College, Fudan University, Shanghai 201508, China
⁵Department of Medical Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea

E-mail:,

Resource and time consuming difficult procedures involving the preparation of antigen surface functionalized nanoparticles mimicking viruses for optimum immunogenicity have been the challenges for their actual application. In this context, we developed stable polymeric particles functionalized with Ni2+-NTA via the combination of RAFT emulsion polymerization and polymerization-induced selfassembly (RAFT-PISA), and mixed with recombinant H1 tagged with hexa-histidine (His6-H1) for one day to prepare the influenza-mimetic polymeric particles. The RAFT-PISA based polymer particles exhibited good structural stability while the Ni2+-NTA-functionality provided a perfect docking site for the controlled orientation of His6-H1. The His6-H1 protein immobilization with Ni2+-NTA of the particle was studied with time. In addition, we investigated the immune response of the synthesized vaccine against influenza with varying the mixing time. We expect our system would open new avenues for preparation of influenza-mimetic vaccines like by mixing of a ready-made surface-functionalized particle and recombinant hemagglutinin.

Keywords:Virus-mimetic;RAFT-PISA;His-tag;Immune stimulatory;Influenza vaccine

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[Referenced By]

[1] Zhao L, Seth A, Wibowo N, Zhao CX, Mitter N, Yu C, Middelberg AP, Vaccine, 32, 327 (2014)

[2] Lopez-Sagaseta J, Malito E, Rappuoli R, Bottomley MJ, Comput. Struct. Biotechnol. J., 14, 58 (2016)

[3] Klontz KC, Singh N, Expert Rev. Anti Infect. Ther., 13, 69 (2015)

[4] Bachmann MF, Jennings GT, Nat. Rev. Immunol., 10, 787 (2010)

[5] Kanekiyo M, Wei CJ, Yassine HM, McTamney PM, Boyington JC, Whittle JRR, Rao SS, Kong WP, Wang LS, Nabel GJ, Nature, 499(7456), 102 (2013)

[6] Couvreur P, Vauthier C, Pharm. Res., 23, 1417 (2006)

[7] Moghimi SM, Hunter AC, Murray JC, Faseb J., 19, 311 (2005)

[8] Tissot AC, Maurer P, Nussberger J, Sabat R, Pfister R, Ignatenko S, Volk HD, Stocker H, Muller P, Jennings GT, Lancet, 371, 821 (2008)

[9] Pankhurst QA, Connolly J, Jones S, Dobson J, J. Phys. D-Appl. Phys., 36, R167 (2003)

[10] Wang T, Zou M, Jiang H, Ji Z, Gao P, Cheng G, Eur. J. Pharm. Sci., 44, 653 (2011)

[11] Saupe A, McBurney W, Rades T, Hook S, Expert Opin. Drug Deliv., 3, 345 (2006)

[12] Quan FS, Huang C, Compans RW, Kang SM, J. Virol., 81, 3514 (2007)

[13] Roldao A, Mellado MCM, Castilho LR, Carrondo MJ, Alves PM, Expert Rev. Vaccines, 9, 1149 (2010)

[14] Juarez V, Pasolli HA, Hellwig A, Garbi N, Arregui AC, Open Virol. J., 6, 270 (2017)

[15] Goldinger SM, Dummer R, Baumgaertner P, Mihic-Probst D, Schwarz K, Hammann-Haenni A, Willers J, Geldhof C, Prior JO, Kundig TM, Eur. J. Immunol., 42, 3049 (2012)

[16] Chen HW, Huang CY, Lin SY, Fang ZS, Hsu CH, Lin JC, Chen YI, Yao BY, Hu CMJ, Biomaterials, 106, 111 (2016)

[17] Lee C, Jeong J, Lee T, Zhang W, Xu L, Choi JE, Park JH, Song JK, Jang S, Eom CY, Biomaterials, 183, 234 (2018)

[18] Warren NJ, Armes SP, J. Am. Chem. Soc., 136(29), 10174 (2014)

[19] Blanazs A, Madsen J, Battaglia G, Ryan AJ, Armes SP, J. Am. Chem. Soc., 133(41), 16581 (2011)

[20] Zhou W, Qu Q, Xu Y, An Z, ACS Macro Lett., 4, 495 (2015)

[21] Tan J, Sun H, Yu M, Sumerlin BS, Zhang L, ACS Macro Lett., 4, 1249 (2015)

[22] Karagoz B, Esser L, Duong HT, Basuki JS, Boyer C, Davis TP, Polym. Chem., 5, 350 (2014)

[23] Yang PC, Mykhaylyk OO, Jones ER, Armes SP, Macromolecules, 49(18), 6731 (2016)

[24] Chaduc I, Reynaud E, Dumas L, Albertin L, D'Agosto F, Lansalot M, Polymer, 106, 218 (2016)

[25] Lv F, An Z, Wu P, Nat. Commun., 10, 1397 (2019)

[26] Delaittre G, Nicolas J, Lefay C, Save M, Charleux B, Chem. Commun., 614 (2005).

[27] Duret D, Haftek-Terreau Z, Carretier M, Ladaviere C, Charreyre MT, Favier A, Polym. Chem., 8, 1611 (2017)

[28] Zhao W, Gody G, Dong S, Zetterlund PB, Perrier S, Polym. Chem., 5, 6990 (2014)

[29] Eggers S, Abetz V, Polymers, 9, 668 (2017)