Cinnamyl acetate has a wide application in food industries. This work focuses on cinnamyl acetate synthesis with a novel esterase.est(GUZ753), an esterase gene fromGeobacillus uzenensisDSMZ 13551, was first cloned and expressed inPichia pastorisKM71. The optimal activity of Est(GUZ753)towardsp-NP caprylate was at pH 8.0 and 70 degrees C, and the half-life at 70 degrees C was 28 h. Furthermore, Est(GUZ753)showed marked tolerance in methanol. The activity of Est(GUZ753)increased up to 1.16-fold in 90% methanol after 72 h, and the half-life in it was 336 h. The crude fermentation broth of Est(GUZ753)was immobilized directly onto the epoxy resin (Lx-105s), and the immobilized Est(GUZ753)exhibited a 99% conversion for cinnamyl acetate synthesis at a high cinnamyl alcohol concentration in 6 h (cinnamyl alcohol: 1.0 M, enzyme dosage: 3%). These characteristics of Est(GUZ753)indicated its great potential for organic synthesis. Graphic A novel esterase gene fromGeobacillus uzenensisDSMZ 13551 was first cloned and expressed inPichia pastorisKM71. Esterase Est(GUZ753)showed marked thermostability at high temperature and tolerance in methanol. Furthermore, the crude fermentation broth of Est(GUZ753)was immobilized directly onto the epoxy resin, and immobilized Est(GUZ753)exhibited a 99% conversion for cinnamyl acetate synthesis.