A silver nanoparticle (AgNP) producing, Gram stain-positive, aerobic, motile, and rod-shaped novel bacterial strain, designated as MAH-34( T)was isolated from rhizospheric soil of magnolia tree. The colonies were creamy white, smooth, circular, and 0.9-2.0 mm in diameter when grown on R2A agar. Strain MAH-34( T)was found to be able to grow at 10-37 degrees C, at pH 6.0-9.5, and at 0-1% NaCl. The strain showed activity for both catalase, and oxidase tests, and was able to rapid synthesis of AgNPs. The TEM image revealed the spherical shape of biosynthesized AgNPs, and the size was 5 to 15 nm. Based on 16S rRNA gene sequence comparisons, the isolate was shown to be a member of genusPaenibacillus, and the close type strains werePaenibacillus chondroitinusDSM 5051( T)(98.3%),Paenibacillus acerisKUDC4121(T)(98.2%),Paenibacillus nebraskensisJJ-59( T)(97.8%),Paenibacillus alginolyticusDSM 5050( T)(97.6%),Paenibacillus ferrariusCY1(T)(97.4%),Paenibacillus frigoriresistensYIM 016( T)(97.3%), andPaenibacillus pocheonensisGsoil 1138( T)(97.3%). Strain MAH-34( T)had a genome size of 8,647,010 bp. The genomic G + C content was 46.0 mol %. The major isoprenoid quinone was determined as menaquinone-7 (MK-7). The major cellular fatty acids were determined as C-15:0 anteiso, and C-16:0 iso. Based on the DNA-DNA hybridization results, genotypic analysis, chemotaxonomic, and physiological data, strain MAH-34( T)represents a novel species, for which the namePaenibacillus anseongensesp. nov. is proposed, with MAH-34( T)as the type strain (= KACC 19974( T) = CGMCC1.16610( T)).