A bacterium, designated HX2-24( T), was isolated from activated sludge treating pesticide-manufacturing wastewater. Colonies of the strain on nutrient agar were circular, transparent, and colorless. Strain HX2-24( T) shared 98.1% 16S rRNA gene sequence similarity with Extensimonas vulgaris S4(T), and less than 97% similarities with other type strains. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain formed a clade with E. vulgaris S4(T). The major cellular fatty acids were C-16:0, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and C-17:0 cyclo, the major polar lipids were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), aminophospholipid (APL), glycophospholipid (GPL), and aminoglycolipid (AGL). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between HX2-24( T) and E. vulgaris S4(T) were 92% and 41%, respectively. The G + C content of strain HX2-24( T) was 64.4 mol%. Thus, based on the phenotypic, chemotaxonomic, and genotypic characteristics, strain HX2-24( T) represents a novel species in the genus Extensimonas, for which the name Extensimonas perlucida HX2-24( T) sp. nov. is proposed. The type strain is HX2-24( T) (= KCTC 72472( T) = CCTCC AB 2019178( T)).