The structural parameters and average molecular structures of the asphaltenes obtained from the Aguacate oilfield, located in the Golden Lane of Mexico, have been investigated combining experimental analysis and molecular simulation. The average molecular structural parameters of the polycyclic aromatic hydrocarbon (PAH) region, average number of fused aromatic rings (nFAR), average structural isomers in the polydispersity of the PAH core, average architecture, average molecular weight, and substituents in the PAH core have been determined by means of( 13)C single-pulse excitation (SPE) nuclear magnetic resonance (NMR) in combination with C-13 distortionless enhancement by polarization transfer (DEPT)-135 degrees experiments, H-1 NMR, X-ray photoelectron spectroscopy, fluorescence emission, and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. The total energy of the PAH isomers and their fluorescence emission are calculated with density functional theory and ZINDO/S, respectively. The PAH region structural parameters determined are (a) Y-carbons (C-Y) or internal triple bridgehead aromatic carbons, (b) external peripheral aromatic carbon atoms at the junction of two fused rings (C-AP3), (c) aromatic carbon atoms bonded to hydrogen atoms (C-AH), (d) aromatic carbon atoms bonded to heteroatom (C-AX), (e) aromatic carbon atoms bonded to hydrogen at the beta position with respect to the heteroatom (C-AH beta X), and (1) substituted aromatic carbon atoms (CA-Calip). The C-13 NMR chemical shift ranges used for the different structural carbon atoms in the PAH core were obtained from our previous study [Ruiz-Morales, Y.; Miranda-Olvera, A. D.; Portales-Martinez, B.; Dominguez, J. M. Determination of C-13 NMR Chemical Shift Structural Ranges for Polycyclic Aromatic Hydrocarbons (PAHs) and PAHs in Asphaltenes: An Experimental and Theoretical Density Functional Theory Study. Energy Fuels 2019, 33 (9), 7950-7970, DOI : 10.1021/acs.energyfuels.9b00182]. There is overlapping of the C-13 SPE NMR signals of C-AH, C-Y, and C-AP3; therefore, quantitative C-13 DEPT-135 degrees NMR is necessary to determine the average CAH, and this value is subtracted from the quantitative C-13 SPE NMR spectrum, where the signals overlap, to obtain the average number of bridgehead carbons (C-Y and C-AP3). It is concluded that island is the predominant architecture of the Aguacate asphaltene, in accordance with the Yen-Mullins model. The PAH region contains nine fused aromatic rings (9FAR), where one of the rings is a thiophenic ring.