Considering that the anode and cathode in batteries have different heat generation behaviors and that there is almost no technique for measuring the heat generation of the anode and cathode under nondestructive conditions, we proposed a novel in-situ nondestructive temperature measurement technique for acquiring the heat generated by the anode and cathode. To this end, a Swagelok Li-ion battery cell is designed to visualize the temperature of the anode and cathode by using an infrared camera. Compared with the anode's heat generation, the cathode generates more heat at a 0.5 C current. The reversible heat generation of the electrode has an exothermic effect, which could be transformed into an endothermic effect within 0% to 100% depth of discharge (DoD). The irreversible heat generation always has an exothermic effect and decreases during the delithiation process. Therefore, it can be concluded that it is likely that variations in the heat generated by the anode and cathode can be measured by using the proposed nondestructive method. Finally, it is meaningful that the effects of the anode/cathode chemistry and other factors such as C-rate and temperature on the local heat generation will be investigated in future works.