Aims Pseudomonas spp. have been widely studied for their plant growth-promoting effects. However, their capacity to promote lipid accumulation in oilseed crops is not well characterized. In this study, we evaluated the effect of Pseudomonas fluorescens LBUM677 on lipid accumulation in three oilseed crops: soybean (Glycine max), canola (Brassica napus) and corn gromwell (Buglossoides arvensis), a plant of high nutraceutical interest for its accumulation of the omega-3 stearidonic acid. Methods and Results Pot experiments were conducted under controlled conditions where seeds were inoculated or not with LBUM677 and plants were harvested at 4, 8 and 12 weeks. A qPCR assay specifically targeting LBUM677 was used in parallel to correlate LBUM677 soil rhizosphere competency to growth promotion and seed lipid accumulation. Total oil seed content and fatty acid composition were analysed at seed maturity. Results showed that LBUM677 was able to establish itself in the rhizosphere of the three plant species at similar levels, but it differentially increased plant biomass, total oil content and lipid composition in a plant-specific manner. Conclusions Despite some species-specific differences observed in P. fluorescens LBUM677's effect on different crops, the strain appears to be a generalist plant growth-promoting rhizobacteria of oilseed crops. Significance and Impact of the Study LBUM677 shows great potential to be used as an inoculum to promote oil yield and fatty acid accumulation in oilseed crops.