This study investigates the charge history of the Oligocene - Lower Miocene Asmari Formation reservoir at three oilfields (Karanj, Paranj and Parsi) in the southern Dezful Embayment, SW Iran, from microthermometric analyses of hydrocarbon-bearing fluid inclusions. The Asmari Formation reservoir was sampled in seven wells at depths of between 1671.5 and 3248.5 m; samples from three of the wells were found to be suitable for fluid inclusion analyses. The samples were analyzed using an integrated workflow including petrography, fluorescence spectroscopy, Raman microspectroscopy and microthermometry. Abundant oil inclusions with a range of fluorescence colours from near-yellow to near-blue were observed. Based on the fluid inclusion petrography, fluorescence and microthermometry data, two episodes of oil charging into the reservoir were identified: 7 to 3.5 Ma, and 3.5 to 2 Ma, respectively. Fluid inclusions in general homogenized at temperatures between 112 and 398 degrees C and with salinities of 14 to 23 wt.% NaCl equivalent. Based on the burial history, the Albian Kazhdumi and Paleogene Pabdeh Formation source rocks in the study area have not reached the gas generation window. The abundant fluid inclusions containing gas-liquid phase observed in the Asmari samples studied are therefore inferred to have been derived from secondary oil-to-gas cracking which resulted from Late Pliocene uplift.