In the present work, ultrasound was combined with three-phase partitioning (UA-TPP) for the extraction of curcuminoids from Curcuma longa. The effect of various process parameters viz. solvents, irradiation time, ammonium sulphate concentration, ratio of turmeric aqueous slurry to t-butanol, turmeric powder to aqueous ratio, and ultrasonic power along with duty cycle on UA-TPP was examined. The yield of 67.15 mg/g was achieved using UA-TPP in 20 min irradiation time at room temperature (30 +/- 2 degrees C) using 30% (w/v) ammonium sulphate saturation, 1:1 (v/v) turmeric aqueous slurry to t-butanol ratio, 1:30 (w/v) turmeric powder to solvent ratio with 22 kHz frequency, 60 W power and 60% (6 sec ON and 4 sec OFF) duty cycle. The comparison study was done based on extraction yields, time, energy requirement and process cost required by UA-TPP, conventional TPP and Soxhlet. UA-TPP found useful not only to enhance the process but also to improve the antioxidant capacity and anti-inflammatory activity of the extract in comparison with TPP.