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Science, Vol.368, No.6495, 2020
Comment on "RNA-guided DNA insertion with CRISPR-associated transposases"
Strecker et al (Research Articles, 5 July 2019, p. 48) described a system for exploiting a Tn7-type transposon-encoded CRISPR-Cas system to make RNA-guided, programmable insertions. Although this system has great promise, we note that the well-established biochemistry of In7 suggests that the particular system used may insert not only the transposon but also the entire donor plasmid.