화학공학소재연구정보센터
Science, Vol.368, No.6495, 2020
Response to Comment on "RNA-guided DNA insertion with CRISPR-associated transposases"
Rice et al. suggest that the CRISPR-associated transposase ShCAST system could lead to additional insertion products beyond simple integration of the donor. We clarify the outcomes of ShCAST-mediated insertions in Escherictila coli. which consist of both simple insertions and integration of the donor plasmid. This latter outcome can be avoided by use of a 5' nicked DNA donor.