화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.89, 383-391, September, 2020
DOI10.1016/j.jiec.2020.06.007  Export Citation
Green preparation of lipase@Ca3(PO4)2 hybrid nanoflowers using bone waste from food production for efficient synthesis of clindamycin palmitate
Anming Wang†, Xinxin Chen, Jianyun Yu, Ningning Li, Huimin Li, Youcheng Yin1, Tian Xie1, †, and Stephen Gang Wu2
  • College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310014, PR China
  • 1Holistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, PR China
  • 2Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USA
E-mail:,
To prepare enzyme@Ca3(PO4)2 for environmentally friendly biocatalysis, Ca2+ and (PO4)3- were extracted from bone waste by acidification. In the nearly neutralized filtrate, the Ca2+ and (PO4)3- formed a Ca3(PO4)2 sediment that crystallized on a template of Thermomyces lanuginosus lipase (TLL) at 4 ℃ for 24 h, producing enzyme@Ca3(PO4)2-Bone hybrid nanoflowers (hNFs). Clindamycin palmitate was efficiently synthesized by transfer of a palmityl moiety from vinyl palmitate to clindamycin free base using these hNFs as a biocatalyst. At 30 ℃ in petroleum ether (PE) as the solvent, the yield of the TLL hNFcatalyzed reaction was as high as 70.0%. Even at a high temperature (80 ℃), the yield in the hNF-catalyzed reaction was still 52.6%, but no product was detected when using free lipase as the catalyst. Moreover, the hNFs retained 90% of their initial activity after 10 cycles (120 h, 12 h per cycle). This green and sustainable method that utilizes bone waste from food production as the raw source of the inorganic component was facile and efficient, and the system may also be applicable for preparing other enzyme@Ca3(PO4)2 hNFs for industrial applications.
Keywords:Hybrid nanoflowers;Clindamycin palmitate;Lipase TLL;Bone waste;Biocatalyst;Immobilized enzyme
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