화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.89, 436-441, September, 2020
DOI10.1016/j.jiec.2020.06.016  Export Citation
A thiourea-based fluorescent chemosensor for bioimaging hypochlorite
Haeri So, Hangyul Lee, Gyu Dong Lee1, Mingeun Kim2, Mi Hee Lim2, Ki-Tae Kim1, †, and Cheal Kim†
  • Department of Fine Chem., Seoul National University of Science and Technology (SNUT), Seoul 01178, Republic of Korea
  • 1Department of Environmental Engineering, Seoul National University of Science and Technology (SNUT), Seoul 01178, Republic of Korea
  • 2Department of Chem., Korea Advanced Institute of Science and Technology (KAIST), Daejeon 33141, Republic of Korea
E-mail:,
A thiourea-based fluorescent turn-off chemosensor FHC was developed for recognizing hypochlorite. With the addition of hypochlorite to FHC, FHC displayed a specific desulfurization reaction of the thiourea group followed by the fluorescent quenching response. The fluorescent turn-off response of FHC to hypochlorite was very fast to finish within a few seconds. Detection limit of hypochlorite was determined to be 0.43 μM. FHC can be applied for the bioimaging of hypochlorite in both HeLa cells and zebrafish. Response process of FHC to hypochlorite, via the desulfurization of the thiourea moiety, was demonstrated, based on 1H NMR titrations, ESI-mass, fluorescent and UV-vis titrations and DFT calculations.
Keywords:Thiourea;Hypochlorite;Fluorescence Chemosensor;Cells;Zebrafish
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