화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.28, No.12, 1098-1103, November, 2020
DOI10.1007/s13233-020-8155-4  Export Citation
Characterization of Platelet-Rich Plasma/Gellan Gum Hydrogel Composite for Biological Performance to Induce Chondrogenesis from Adipose-Derived Stem Cells
Namyeong Kim, Joo Hee Choi, Min Joung Choi, Jin Su Kim, Wooyoup Kim, Jeong Eun Song, and Gilson Khang†
  • Department of PolymerNano Science & Technology and Polymer Materials Fusion Research Center, Department of Bionanotechnology and Bio-Convergence Engineering, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeonbuk 54896, Korea
E-mail:
In this experiment, hydrogel composite was developed with gellan gum (GG) and platelet-rich plasma (PRP) to induce chondrogenesis of adipose-derived stem cells (ADSCs) in the hydrated environment. FTIR, SEM, and compression test were carried out to characterize the fabricated material. Biocompatibility was analyzed by MTT assay. The morphology of the encapsulated ADSCs was observed under SEM. Chondrogenesis was examined by PCR. The chemical and morphological analysis displayed the appearance of PRP in the matrix. The mechanical property of the hydrogel decreased as the PRP was loaded but viability and chondrogenesis were significantly increased with the proper amount of the PRP. Overall, the composite of the appropriate incorporation of PRP in the GG can enhance the microenvironment for chondrogenesis and expected to have great potential in tissue engineering.
Keywords:adipose-derived stem cells;gellan gum;platelet-rich plasma;chondrogenesis
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