Optimal Voltage and Electrical Pulse Conditions for Electrical Ablation to Induce Immunogenic Cell Death (ICD)

Eun-Jin Go; Dasom Yang; WonHyoung Ryu; Hong Jae Chon; Chan Kim; Kyung-Soon Park; Dong-Hyun Kim; Dong Keun Han; Wooram Park

Journal of Industrial and Engineering Chemistry, Vol.94, 225-232, February, 2021

DOI10.1016/j.jiec.2020.10.038 Export Citation

Optimal Voltage and Electrical Pulse Conditions for Electrical Ablation to Induce Immunogenic Cell Death (ICD)

Eun-Jin Go^{1, 2}, Dasom Yang³, WonHyoung Ryu³, Hong Jae Chon⁴, Chan Kim⁴, Kyung-Soon Park², Dong-Hyun Kim^{5, 6}, Dong Keun Han^{2, †}, and Wooram Park^{1, †}

¹Department of Biomedical-Chemical Engineering, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-Si, Gyeonggi-do 14662, Republic of Korea
²Department of Biomedical Science, CHA University, 335 Pangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13488, Republic of Korea
³Department of Mechanical Engineering, Yonsei University, Seoul 03722, Republic of Korea
⁴Medical Oncology, CHA Bundang Medical Center, CHA University Medical School, 59 Yatap-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13496, Republic of Korea
⁵Department of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
⁶Robert H. Lurie Comprehensive Cancer Center, Chicago, IL 60611, USA

E-mail:,

Electrical ablation (EA) is a non-thermal ablation technique that causes less damage to the tissue surrounding the targeted area than conventional thermal ablation. EA induces immunogenic cell death (ICD) and can potentially synergize with cancer immunotherapy. In this study, the optimal voltage and pulse conditions for the induction of ICD were identified. Finite element analysis was used to estimate the ablation zone under a variety of voltage and pulse conditions. Cancer cells were cultured in vitro under two- and three-dimensional conditions to assess cell viability under different voltage and pulse conditions. Additionally, the expression of damage-associated molecular pattern (DAMP) markers of ICD was measured to identify an optimal voltage condition. Tumor volume, body weight, and survival after EA treatment were measured in vivo to identify the optimal pulse conditions. The optimal conditions to induce ICD by EA identified in this study are suitable to be combined with cancer immunotherapy.

Keywords:Tumor ablation;Electrical ablation;Immunogenic cell death;Damage-associated molecular pattern;molecules;Cancer immunotherapy

[References]

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[2] Davalos RV, Mir L, Rubinsky B, Ann. Biomed. Eng., 33, 223 (2005)

[3] Narayanan G, Irreversible electroporation, Thieme Medical Publishers, pp.349 2015.

[4] Davalos RV, Rubinsky B, Int. J. Heat Mass Transf., 51(23-24), 5617 (2008)

[5] Garcia PA, Rossmeisl JH, Neal RE, Ellis TL, Olson JD, Henao-Guerrero N, Robertson J, Davalos RV, J. Membr. Sci., 236, 127 (2010)

[6] Scheffer HJ, Nielsen K, de Jong MC, van Tilborg AA, Vieveen JM, et al., J. Vasc. Interv. Radiol., 25, 997 (2014)

[7] Huang KW, Yang PC, Pua U, Kim MD, Li SP, Qiu YD, Song TQ, Liang PC, J. Surg. Oncol., 118, 31 (2018)

[8] Belfiore G, Belfiore NP, Reginelli A, Capasso R, Romano F, Ianniello GP, Cappabianca S, Brunese L, Med. Oncol., 34, 38 (2017)

[9] Bhutiani N, Agle S, Li Y, Li S, Martin RC, J. Surg. Oncol., 114, 181 (2016)

[10] Zhao J, Qiao Y, Zhou M, Wallace M, Gupta S, Li C, Melancon MP, ACS Macro Lett, 4, 1081 (2015)

[11] Mouli SK, Tyler P, McDevitt JL, Eifler AC, Guo Y, Nicolai J, Lewandowski RJ, Li W, Procissi D, Ryu RK, ACS Nano, 7, 7724 (2013)

[12] Zhao J, Wen X, Tian L, Li T, Xu C, Wen X, Melancon MP, Gupta S, Shen B, Peng W, Nat. Commun., 10, 1 (2019)

[13] Lin M, Alnaggar M, Liang S, Wang X, Liang Y, Zhang M, Chen J, Niu L, Xu K, Oncotarget, 8, 101795 (2017)

[14] He C, Huang X, Zhang Y, Lin X, Li S, Clin. Transl. Med., 10, e39 (2020)

[15] Polajzer T, Jarm T, Miklavcic D, Radiol. Oncol., 54, 317 (2020)

[16] Obeid M, Tesniere A, Ghiringhelli F, Fimia GM, Apetoh L, Perfettini JL, Castedo M, Mignot G, Panaretakis T, Casares N, Nat. Med., 13, 54 (2007)

[17] Tesniere A, Apetoh L, Ghiringhelli F, Joza N, Panaretakis T, Kepp O, Schlemmer F, Zitvogel L, Kroemer G, Curr. Opin. Immunol., 20, 504 (2008)

[18] Martins I, Wang Y, Michaud M, Ma Y, Sukkurwala A, Shen S, Kepp O, Metivier D, Galluzzi L, Perfettini J, Cell Death Differ., 21, 79 (2014)

[19] Sweeney EE, Cano-Mejia J, Fernandes R, Small, 14, 180067 (2018)

[20] Nam J, Son S, Ochyl LJ, Kuai R, Schwendeman A, Moon JJ, Nat. Commun., 9, 1 (2018)

[21] Ma Y, Zhang Y, Li X, Zhao Y, Li M, Jiang W, Tang X, Dou J, Lu L, Wang F, ACS Nano, 13, 11967 (2019)

[22] Wen Y, Chen X, Zhu X, Gong Y, Yuan G, Qin X, Lin J, ACS Appl. Mater. Interfaces, 11, 43393 (2019)

[23] Sung CK, Kim HB, Jung JH, Baik KY, Moon KW, Kim HS, Yi JH, Technol. Cancer Res. Treat., 16, 488 (2017)

[24] Zhang Y, Guo Y, Ragin AB, Lewandowski RJ, Yang GY, Nijm GM, Sahakian AV, Omary RA, Larson ACJR, Radiology, 256, 424 (2010)

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[26] Jiang C, Shao Q, Bischof J, Ann. Biomed. Eng., 43, 887 (2015)

[27] Jung B, Hong S, Kim SC, Hwang C, Tissue Eng. Regen. Med., 15, 275 (2018)

[28] Lee J, Lee SH, Lee BK, Park SH, Cho YS, Park Y, Tissue Eng. Regen. Med., 15, 403 (2018)

[29] Park KM, Park KD, Tissue Eng. Regen. Med., 15, 547 (2018)

[30] Krysko DV, Garg AD, Kaczmarek A, Krysko O, Agostinis O, Vandenabeele P, Nat. Rev. Cancer, 12, 860 (2012)

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[32] Cheung W, Kavnoudias H, Roberts S, Szkandera B, Kemp W, Thomson KR, Technol. Cancer Res. Treat., 12, 233 (2013)

[33] Cannon R, Ellis S, Hayes D, Narayanan G, Martin RC, J. Surg. Oncol., 107, 544 (2013)

[34] Love MR, Palee S, Chattipakorn SC, Chattipakorn N, J. Cell. Physiol., 233, 1860 (2018)

[35] Kuzyk PR, Schemitsch EH, Indian J. Orthop., 43, 127 (2009)

[36] Xiang Y, Liu H, Yan T, Zhuang Z, Jin D, Peng Y, Neural Regen. Res., 9, 243 (2014)

[37] Dollinger M, Beyer LP, Haimerl M, Niessen C, Jung EM, Zeman F, Stroszczynski C, Wiggermann P, Diagn. Interv. Radiol., 21, 471 (2015)

[38] Ball C, Thomson KR, Kavnoudias H, Anesth. Analg., 110, 1305 (2010)

[39] Park W, Heo YJ, Han DK, Biomater. Res., 22, 1 (2018)

[40] Park W, Song KH, Lim J, Park CG, Doh J, Han DK, Int. Mater. Rev., 65, 445 (2020)