Silk fibroin microneedles fabricated by digital light processing 3D printing

Donghyeok Shin; Jinho Hyun

Journal of Industrial and Engineering Chemistry, Vol.95, 126-133, March, 2021

DOI10.1016/j.jiec.2020.12.011 Export Citation

Silk fibroin microneedles fabricated by digital light processing 3D printing

Donghyeok Shin^{1, 2}, and Jinho Hyun^{1, 2, †}

¹Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul 08826, Republic of Korea
²Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Seoul 08826, Republic of Korea

E-mail:

In this report, one-step 3D structuring of protein-based microneedles is proposed using digital light processing (DLP) 3D printing. Such a technique is advantageous in direct 3D construction of molecules dissolved in aqueous solution at relatively low concentration. The DLP printing of silk fibroin (SF) as a safe protein-based microneedle is challenging, and use of riboflavin, an enzyme that acts as a photoinitiator, reduces the toxicity in biomedical applications. An anti-aliasing process produces SF microneedles with oblique features, while dehydration of the printed hydrogel enhances the sharpness of printed features. The flexibility of an SF microneedle array pad is investigated, and the delivery of molecules to the skin is verified using rhodamine B fluorescence dye.

Keywords:Silk fibroin;Digital light processing;3D printing;Microneedle;Riboflavin;Hydrogel

[References]

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[2] Sharma S, Hatware K, Bhadane P, Sindhikar S, Mishra DK, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 103, 109717 (2019)

[3] Liu JL, Feng YH, Zhang XP, Zhu DD, Zhang LQ, Guo XD, J. Ind. Eng. Chem., 84, 280 (2020)

[4] Zhang LQ, Zhang XP, Hao YY, Zhang BL, Guo XD, J. Ind. Eng. Chem., 88, 241 (2020)

[5] Chandrasekaran S, Brazzle JD, Frazier AB, J. Microelectromech. S, 12, 281 (2003)

[6] Gardeniers HJGE, Luttge R, Berenschot EJW, de Boer MJ, Yeshurun SY, Hefetz M, van’t Oever R, van den Berg A, J. Microelectromech. S, 12, 855 (2003)

[7] Wilke N, Mulcahy A, Ye SR, Morrissey A, Microelectron. J., 36, 650 (2005)

[8] Park JH, Allen MG, Prausnitz MR, J. Control. Release, 104, 51 (2005)

[9] Takahashi H, Heo YJ, Arakawa N, Kan T, Matsumoto K, Kawano R, Shimoyama I, Microsyst. Nanoeng., 2, 16049 (2016)

[10] Lu Y, Mantha SN, Crowder DC, Chinchilla S, Shah KN, Yun YH, Wicker RB, Choi JW, Biofabrication, 7, 045001 (2015)

[11] Johnson AR, Caudill CL, Tumbleston JR, Bloomquist CJ, Moga KA, Ermoshkin A, Shirvanyants D, Mecham SJ, Luft JC, DeSimone JM, PLoS One, 11, e01625 (2016)

[12] Lim SH, Ng JY, Kang L, Biofabrication, 9, 015010 (2017)

[13] Johnson AR, Procopio AT, 3D Print. Med., 5, 2 (2019)

[14] Lin H, Zhang D, Alexander PG, Yang G, Tan J, Cheng AW, Tuan RS, Biomaterials, 34, 331 (2013)

[15] Sun C, Fang N, Wu DM, Zhang X, Sens. Actuators A-Phys., 121, 113 (2005)

[16] Park IB, Ha YM, Lee SH, Sens. Actuators A-Phys., 167, 117 (2011)

[17] Narayanan SP, Raghavan S, Int. J. Adv. Manuf. Technol., 93, 407 (2016)

[18] Li J, Liu B, Zhou Y, Chen Z, Jiang L, Yuan W, Liang L, PLoS One, 12, e01720 (2017)

[19] Moga KA, Bickford LR, Geil RD, Dunn SS, Pandya AA, Wang YP, Fain JH, Archuleta CF, O'Neill AT, DeSimone JM, Adv. Mater., 25(36), 5060 (2013)

[20] Yucel T, Lovett ML, Kaplan DL, J. Control. Release, 190, 381 (2014)

[21] You XQ, Chang JH, Ju BK, Pak JJ, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 31, 1632 (2011)

[22] Stinson JA, Raja WK, Lee S, Kim HB, Diwan I, Tutunjian S, Panilaitis B, Omenetto FG, Tzipori S, Kaplan DL, ACS Biomater. Sci. Eng., 3, 360 (2017)

[23] Deng S, Wu J, Dickey MD, Zhao Q, Xie T, Adv. Mater., 31, e19039 (2019)

[24] Lee HJ, Shin DH, Shin SC, Hyun JH, J. Ind. Eng. Chem., 89, 119 (2020)

[25] Hong H, Seo YB, Kim DY, Lee JS, Lee YJ, Lee H, Ajiteru O, Sultan MT, Lee OJ, Kim SH, Park CH, Biomaterials, 232, 119679 (2020)

[26] Cui X, Soliman BG, Alcala-Orozco CR, Li J, Vis MAM, Santos M, Wise SG, Levato R, Malda J, Woodfield TBF, Rnjak-Kovacina J, Lim KS, Adv. Healthc. Mater., 9, e19016 (2020)

[27] Kwak HJ, Shin SC, Lee HJ, Hyun JH, J. Ind. Eng. Chem., 72, 232 (2019)

[28] Applegate MB, Partlow BP, Coburn J, Marelli B, Pirie C, Pineda R, Kaplan DL, Omenetto FG, Adv. Mater., 28(12), 2417 (2016)

[29] Partlow BP, Applegate MB, Omenetto FG, Kaplan DL, ACS Biomater. Sci. Eng., 2, 2108 (2016)

[30] Batchelor RR, Kwandou G, Spicer PT, Stenzel MH, Polym. Chem., 8, 980 (2017)

[31] Ibusuki S, Halbesma GJ, Randolph MA, Redmond RW, Kochevar IE, Gill TJ, Tissue Eng., 13, 1995 (2007)

[32] Park HJ, Choi BG, Hu J, Lee M, Acta Biomater., 9, 4779 (2013)

[33] Wray LS, Hu X, Gallego J, Georgakoudi I, Omenetto FG, Schmidt D, Kaplan DL, J. Biomed. Mater. Res. B Appl. Biomater., 99, 89 (2011)

[34] Min KT, Kim SK, Kim SH, Proc. Natl. Acad. Sci. U. S. A., 114, 6185 (2017)

[35] Raia NR, Partlow BP, McGill M, Kimmerling EP, Ghezzi CE, Kaplan DL, Biomaterials, 131, 58 (2017)

[36] Kang HW, Park JH, Cho DW, Sens. Actuators A-Phys., 178, 223 (2012)

[37] Zhou C, Chen Y, J. Manuf. Process., 14, 107 (2012)

[38] Yang SY, O’Cearbhaill ED, Sisk GC, Park KM, Cho WK, Villiger M, Bouma BE, Pomahac B, Karp JM, Nat. Commun., 4, 1702 (2013)

[39] Chen W, Tian R, Xu C, Yung BC, Wang G, Liu Y, Ni Q, Zhang F, Zhou Z, Wang J, Niu G, Ma Y, Fu L, Chen X, Nat. Commun., 8, 1777 (2017)

[40] Kim MJ, Park SC, Rizal B, Guanes G, Baek SK, Park JH, Betz AR, Choi SO, Front. Bioeng. Biotechnol., 6, 54 (2018)