A hierarchical Beta zeolite has been successfully mass-prepared hydrothermally and dynamically in a pilot-scale autoclave by an economical and simple process with low-cost raw materials. The characterization results indicated that the pilot-scale hierarchical Beta zeolite has the same textural, structural, and acidic properties like the bench-scale sample. Moreover, the catalyst composed of the pilot-scale hierarchical H-Beta zeolite showed higher activity and selectivity to the middle distillate than that containing microporous Beta zeolite in catalytic hydrocracking of vacuum gas oil (VGO) evaluated by an industrial pilot installation in running of 3000 h, due to the increased accessibility and the reduction of diffusion constraint of large molecules to acid sites in the Beta zeolite framework.