A Gram-staining-negative, halophilic, aerobic, oval-shaped or vibrio-shaped, motile by a polar flagellum strain, designated YL5-2(T), was isolated from natural saline-alkaline wetland soil of Binhai new district, Tianjin, China. Strain YL5-2(T) grew optimally at 35 degrees C, pH 7.5-8.0, and in the presence of 10-25% (w/v) NaCl on MA medium. Phylogenetic analyses based on 16S rRNA gene sequences showed that the isolate belonged to the genus Halovibrio and exhibited high sequence similarity of 97.7% to Halovibrio variabilis DSM 3050(T). The sole respiratory ubiquinone of strain YL5-2(T) is Q-9, and the dominant fatty acids were C-18:1 omega 9c, C-16:0, C-19:0 cyclo omega 8c, and Summed Feature 8. The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC), and lipid (L). The DNA G+C content of the strain was 62.1 mol%. The average nucleotide identity (ANI) based on whole genome sequences of strain YL5-2(T) and Halovibrio variabilis DSM 3050(T) was 93.85%, and the dDDH value between these two strains was determined to be 52.0%. Phenotypic, chemotaxonomic, phylogenetic, and genomic analyses suggested that strain YL5-2(T) represent a novel species of the genus Halovibrio, for which the name Halovibrio salipaludis sp. nov. is proposed. The type strain is YL5-2(T) (=KCTC 52852(T)=ACCC 19971(T)).