Strain HX-7-9(T)was isolated from the activated sludge collected from the outlet of the biochemical treatment facility of agricultural chemical plant in Nanjing, Jiangsu province, PR China. Strain HX-7-9(T)is Gram staining-negative, rod-shaped, non-spore-forming and flagellated. The 16S rRNA gene-based phylogenetic analysis indicate that strain HX-7-9(T)belongs to the genusCrenobacter, moderately related toCrenobacter luteusYIM-78141(T)(94.8% similarity). The genomic DNA G+C content of the strain was 67.5 mol%. Strain HX-7-9(T)was able to grow at 16-45 degrees C (optimum at 37 degrees C), at pH 6.0-8.0 (optimum at pH 7.0) and with 0-1% (w/v) NaCl (optimum at 0). Predominant fatty acid constituents were C(16:0)and summed feature 3 (C-16:1 omega 7cand/or C-16:1 omega 6c). The respiratory ubiquinone was Q-8. The polar lipid profile is composed of diphosphatidylglycerol (DPG), phosphatidylmonomethylethanolamine (PME), phosphatidylethanolamine (PE), phospholipids (PL), glycolipid (GL) and aminophospholipid (APL). The ANI and dDDH values obtained between the genomes of HX-7-9(T)andC. luteusYIM-78141(T)were 79.8 and 19.1%, respectively. On the basis of data from phenotypic, chemotaxonomic and genotypic analysis, strain HX-7-9(T)represents a novel species of the genusCrenobacter, for which the nameCrenobacter caenisp. nov. is proposed. The type strain is HX-7-9(T)(= KCTC 72654(T) = CCTCC AB 2019349(T)).