The purpose of this study was to elaborate the characteristics ofPseudomonassp. Cr13, including physiological and biochemical characteristics, optimization of growth conditions, minimum inhibitory concentration of Cr(6+)and resistance to other heavy metals, removal efficiency of Cr6+, and antibiotics sensitivity. A strainPseudomonassp. Cr13 was screened from mine-contaminated soils, which could tolerate high concentration of Cr6+(up to 250 mg l(-1)) and Cd2+(50 mg l(-1)). The optimum pH, NaCl concentration, and temperature for growth were 6, 10% NaCl, and 30 degrees C, respectively. The removal efficiency of Cr(6+)by strainPseudomonassp. Cr13 was studied. The removal efficiency of Cr(6+)decreased with the increased concentration of Cr6+. Under the optimal conditions, the maximum of the removal rate can reach up to 94.26% in contaminated soils. In addition, antibiotics sensitivity of this strain was investigated. It was found that this strain was sensitive to nine types of antibiotics, which would lay a good foundation for the choice of selective marker in genetic engineering modification of this strain. The results in this article would lay a good foundation for the bioremediation of heavy metals pollution in the future.Pseudomonassp. Cr13 can tolerate high concentration of Cr(6+)and partially remove Cr6+, which make Cr13 an attractive option for the bioremediation of heavy metal chromium (Cr). Our findings suggest thatPseudomonassp. Cr13 is a potential bacterium with the ability of bioremediation of heavy metal Cr.