An exopolysaccharide (EPS-I) having the molecular weight similar to 2.6 x 10(5) Da, was isolated from a Zinc resistant strain ofEnterococcus faecalisfrom costal area. The exopolysaccharide consists of D-mannose, D-glucose, and L-fucose in molar ratio of 9:4:1. The monosaccharide units in the EPS-1 were determined through chemical (total acid hydrolysis and methylation analysis) and spectroscopic (FTIR and(1)H NMR experiment) analysis. The mannose-rich EPS-1 showed total antioxidant activity (1 mg mL(-1)of EPS-I as functional as approximately to 500 +/- 5.2 mu M of ascorbic acid) and Fe(2+)metal ion chelation activity (EC50 = 405.6 mu g mL(-1)) and hydroxyl radical scavenging activity (EC50 = 219.5 mu g mL(-1)). The in vitro cytotoxicity experiment of EPS-I against cervical carcinoma cell line, HeLa cells showed strong cytotoxic effect (LC50 = 267.3 mu g mL(-1)) and at that concentration, it found almost nontoxic against normal healthy cells (HEK-293).