A strain SDU10 was isolated from swine manure compost and identified asPseudomonas stutzeriSDU10. It demonstrated excellent capability in NH4+-N removal. Optimal conditions of NH4+-N removal were determined, which were sodium acetate as the optimal carbon source, carbon to nitrogen (C/N) ratio of 10, temperature of 30 degrees C, pH of 7.0. Especially,P. stutzeriSDU10 could remove high concentration NH4+-N of 1500.0 and 2000.0 mg/l in 120 h with the NH4+-N removal rates of 91.1% and 61.6%, respectively. In batch experiments, the highest NH4+-N removal rate of 97.6% and chemical oxygen demand (COD) removal rate of 94.2% were obtained at initial C/N ratio 10 during piggery wastewater treatment usingP. stutzeriSDU10. Results showed thatP. stutzeriSDU10 had the potential for treatment of wastewater of high NH4+-N concentration.