Domains of unknown function protein family 1517 (DUF1517) in Ammopiptanthus mongolicus, could be induced by abiotic stresses, whose upstream regulatory sequence might be an ideal source of abiotic-induced promoter. In this study, a 1026-bp promoter of AmDUF1517 from A. mongolicus was cloned. Five deletion fragments (Full, Q1-Q4) of different length of the AmDUF1517 promoter were fused with the beta-glucuronidase (GUS) reporter and transformed into Arabidopsis thaliana. The deletion analysis showed that sequences Full, Q1 and Q3 responded well to mannitol, NaCl and 4 degrees C stresses, while Q2 and Q4 segments did not. The Q3 fragment (280 bp; -280 to -1 bp) showed the highest promoter activity under normal and mannitol, NaCl and 4 degrees C conditions. The result suggested that Q3 in the AmDUF1517 gene promoter could be a new source of induced promoters for abiotic resistance breeding in plant genetic engineering. (C) 2020, The Society for Biotechnology, Japan. All rights reserved.