In this report, a LipL32 gene based nanofabricated electrochemical sensor for the detection of Leptospira interrogans has been developed using carboxylated multiwalled carbon nanotubes with gold nanoparticles (c-MWCNTs/nanoAu) electrode and graphene quantum dots (GQDs). The c-MWCNTs/nanoAu electrode was linked to GQDs using 4-aminothiophenol (ATP). The surface modifications on the electrode surface were delineated using Raman spectroscopy and field emission scanning electron microscopy (FE-SEM). 50-Amino (NH2) labeled single stranded DNA (ssDNA) probe was immobilized on the surface of c-MWCNTs/nanoAu/ATP/GQD composite electrode. The electrochemical changes of the developed sensor after hybridization with single stranded complementary DNA of L. interrogans were analyzed by differential pulse voltammetry (DPV) using 1 mM methylene blue. The sensor showed good linearity with complementary ssDNA concentration ranging from 0.37 to 12 ng/mu l. The sensor exhibited high specificity to L. interrogans and showed good reproducibility when stored at 4 degrees C. (C) 2019, The Society for Biotechnology, Japan. All rights reserved.