Pollution of tetracyclines (TCs) in swine wastewater has been a critical concern worldwide. Notably, multiple anions (e.g. PO43-, NO3-) coexist in the actual environments, which could significantly influence the TCs removal. In the current study, MgAl layered double oxide (MgAl-LDO) was adopted for investigating the TC removal performance with/without PO43- or NO3-. In all systems, the adsorption performance exhibited two different approaches between low and high TC concentrations. In the single system, pseudo-second-order and the Freundlich model fitted well to the equilibrium adsorption data when TC concentration was below 125 mg.L-1, while the pseudo-first-order and the linear model could describe the removal process at high TC concentration (>125 mg.L-1). The maximum adsorption capacity was 83.56 mg.g(-1). In the co-existing system, the adsorption capacity was slightly enhanced when TC concentration below 150 mg.L-1 however was inhibited at high concentration (>150 mg.L-1). Combined with the characterization analyses, the interaction mechanism at low concentration was primarily surface adsorption on reconstructed LDH from LDO in the TC-alone system. It is worth mention that both PO43- and NO3- facilitated the formation of LDH via rehydration of LDO which enhanced surface adsorption in the co-existing system. At high TC concentration, the formation of tetracycline-metal complexes played a dominant role in TC removal in the single system, whereas diminished complexation in the binary system led to the decreased TC removal. This study provides a theoretical and practical guidance for MgAl-LDO on the efficient remediation of actual tetracyclines wastewater. (C) 2020 Published by Elsevier Inc.