This experiment aims to study the performance of multilayered transparent wood (MLTW) under the condition of partial delignification, including the effect of lamination structure between layers and delignification process on the properties of MLTW. New Zealand pine (Pinus radiata D.Don) and Basswood (Tilia) were selected. The acid method was used to remove part lignin for preparing wood templates with natural wood color and texture, and then the polymer impregnated wood templates. During the lamination process, three wood templates were laminated by parallel direction (MLTW parallel to) or perpendicular direction (MLTW perpendicular to) to prepare for MLTW. Under macro- and microtest, it can be seen that the laminate structure has a certain impact on the mechanical properties and light transmittance of MLTW, and MLTW can effectively reduce the anisotropy of single-layer one with the same thickness. MLTW is suitable for home decoration and structure materials and has broad application prospects.