In this study, we report a facile preparation of an organic solvent-resistant membrane through the formation of urethane bonds between polycarbonate and polyethyleneimine groups. The modified membrane was further cross-linked with 1,4-butanediol diglycidyl ether (BDG) to enhance its solvent resistance as well as its thermal and mechanical stability. The cross-linked polycarbonate membranes exhibited improved solvent resistance with various organic solvents, giving a maximum swelling degree of 6%. It also showed better mechanical and thermal stability, as well as excellent permeance and rejection performance. This study demonstrates BDG as an attractive cross-linker for polycarbonate microfiltration membranes to transform them toward organic solvent filtration applications.