The manipulation technology of particles is significant in drug screening, disease detection and treatment, etc. Here, we reported the multidomain oriented particle chains based on a spatial electric field and their optical application. According to the differences in the dielectric behavior of particles, the preparation of multidomain oriented particle chains in the gel was successfully realized by using the dielectrophoretic force and electroosmotic rotation. This provides a new idea for manufacturing multistructure, multilayer, and multifunctional intelligent response materials. In addition, the factors affecting the alignment height of the particles in the gel were discussed, which was the basis for the preparation of bilayer particle chains. As an example of structural hierarchy, particle assembly has broad application prospects in optoelectronic devices and soft robots.