The acetogenins are strongly bioactive natural compounds present in the bark, roots, leaves, and seeds of many Annonaceae plants. They are modified fatty acids and their cytotoxicities have been determined for different biological models including the in vitro growth inhibition of several human cancer cell lines. Very low acetogenin yield (< 0.1 g%) has been found previously in native phytobiomass, and we have now investigated the nonpredatory exploitation of the seeds as acetogenin sources characterizing the seed triacylglycerols (dominant fraction; > 90% of the whole lipid extracts) as potential valuable by-products. Supercritical fluid extraction (SFE) was utilized as an alternative for both kinds of lipid biomass, acetogenins and fats, and manipulation of Annona explants for obtaining in vitro callus and/or new plants.