Use of nano-additives in biofuels is an important research and development topic for achieving optimum engine performance with reduced emissions. In this study, rice bran oil was converted into biodiesel and graphene oxide (GO) nanoparticles were infused into biodiesel-diesel blends. Two blends containing (i) 5% biodiesel, 95% diesel and 30 ppm GO (B5D95GO30) and (ii) 15% biodiesel, 85% diesel and 30 ppm GO (B15D85GO30) were prepared. The fuel properties like heating value, kinematic viscosity, cetane number, etc. of the nanoadditives-biodiesel-diesel blends (NBDB) were measured. Effects of injection timing (IT) on the performance, combustion and emission characteristics were studied. It was observed that both B15D85GO30 and B5D95GO30 blends at IT23 degrees gave up to 13.5% reduction in specific fuel consumption. Compared to diesel, the brake thermal efficiency was increased by 7.62% for B15D85GO30 at IT23 degrees and IT25 degrees. An increase in IT from 23 degrees to 25 degrees deteriorated the indicated thermal efficiency by 6.68% for B15D85GO30. At maximum load condition, the peak heat release rates of NBDB were found to be lower than the pure diesel at both IT. The CO, CO2 & NOx emissions were reduced by 2-8%. The study concluded that B15D85GO30 at IT23 degrees gave optimum results in terms of performance, combustion and emission characteristics. (C) 2020 Elsevier Ltd. All rights reserved.