One-Pot electrochemical fabrication of high performance amperometric enzymatic biosensors using polypyrrole and polydopamine

Mingyu Lee; Semin Kim; Minsoo Jang; Hyun S. Park; Jae Young Lee

Journal of Industrial and Engineering Chemistry, Vol.97, 316-325, May, 2021

DOI10.1016/j.jiec.2021.02.009 Export Citation

One-Pot electrochemical fabrication of high performance amperometric enzymatic biosensors using polypyrrole and polydopamine

Mingyu Lee, Semin Kim, Minsoo Jang, Hyun S. Park¹, and Jae Young Lee^†

School of Materials Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju, 61005, Republic of Korea
¹Korea Institute of Science and Technology, Fuel Cell Research Center, Hwarangro 14-gil 5, Seoul 02792, Republic of Korea

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We report a novel strategy to offer a high-performance biosensor platform by fabricating an electrically conductive and adhesive electrode composed of polypyrrole (PPy)/polydopamine (PDA) with enzymes (i.e., glucose oxidase (GOx) or lactate oxidase (LOx)) by one-step electrochemical coating. We focused on electrochemical glucose sensing using PDA/PPy and GOx as a model biosensor. PDA/PPy/GOx biosensor was highly sensitive (28 times higher) compared to the PPy/GOx, which appeared to result from enhanced GOx immobilization and efficient signal transduction by PDA/PPy. A selective layer of polyphenol (PPh) on the PDA/PPy/GOx successfully eliminated possible interference. Finally, the GOx biosensor displayed a good sensitivity of 22.15 μ A mM-1cm-2, a short response time of 5-6 s, a linear range up to 5.0 mM, and a detection limit of 138 mM glucose (R2 = 0.995). Human blood serum tests accurately measured glucose concentration. PPh-layered PDA/PPy/GOx biosensor maintained excep- tional stability over 90 days (93.9%). Furthermore, we fabricated a lactate biosensor (PDA/PPy/LOx) by electrochemical PDA/PPy deposition with LOx and demonstrated the significant improvement of a lactate sensing ability compared to PPy/LOx. This facile fabrication strategy involving PDA/PPy and enzymes will serve as an attractive platform for highly sensitive and stable biosensors, such as urate and bilirubin detection.

Keywords:Biosensor;Glucose;Enzymatic;Electrochemical;Polypyrrole;Polydopamine

[References]

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[4] Cosnier S, Biosens. Bioelectron., 14, 443 (1999)

[5] Sassolas A, Blum LJ, Leca-Bouvier BD, Biotechnol. Adv., 30, 489 (2012)

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