화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Journal of Industrial and Engineering Chemistry, Vol.100, 288-295, August, 2021
DOI10.1016/j.jiec.2021.05.010  Export Citation
Covalent immobilization of carbonic anhydrase on amine functionalized alumino-Siloxane aerogel beads for biomimetic sequestration of CO2
Shamna I, Soon Kwan Jeong1, †, and Margandan Bhagiyalakshmi†
  • Department of Chemistry, Central University of Kerala, Kasaragod 671320 India
  • 1Fine Dust Research Center, Korea Institute of Energy Research, Daejeon 34129, Republic of Korea
E-mail:,
Biological CO2 sequestration using carbonic anhydrase is the most promising and eco-friendly technique in CO2 capture technology. The present study entails biomimetic CO2 capture using Carbonic Anhydrase from bovine erythrocytes (BCA) immobilized on versatile porous alumino-siloxane hybrid aerogel beads through covalent attachment. Alumino-siloxane hybrid aerogel beads (Al/Si) were functionalized with (3-Aminopropyl)- trimethoxysilane to achieve effective loading of the enzyme. Amine functionalized hybrid beads (Al/Si-NH2) were characterized by X-Ray Diffraction (XRD), Fourier Transform Infrared (FTIR), Field Emission-Scanning Electron Microscope (FE-SEM), and Brunauer.Emmett.Teller (BET). The biocatalytic activity of BCA immobilized on amine-functionalized alumino-siloxane aerogel beads (BCAAl/ Si-NH2) was estimated by hydrolysis of p-nitrophenyl acetate (p-NPA). Kinetic parameter, km and kcat / km values of BCA-Al/Si-NH2 hybrid beads were found to be comparable with the free enzyme. BCA immobilized Al/Si-NH2 hybrid beads possess thermal stability, pH stability, and storage stability and retained almost 90% of activity over ten consecutive reaction cycles. The carbonation capacity of BCA-Al/ Si-NH2 hybrid beads is found to be 12 mg of CaCO3/17 μg of BCA. Thus, BCA immobilized on aminefunctionalized alumino-siloxane aerogel beads has been found to directly promote the absorption of CO2 and store the captured CO2 as inert solid carbonates.
Keywords:Carbonic anhydrase;Immobilization;Alumino-siloxane aerogel beads;CO2 capture;Biomimetic CO2 sequestration
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